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KMID : 0355819960200020127
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Korean Journal of Oral Anatomy
1996 Volume.20 No. 2 p.127 ~ p.140
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DIFFERENTIAL EFFECT OF PLATELET-DERIVED GROWTH FACTOR AND INSULIN-LIKE GROWTH FACTOR ON PROLIFERATION OF PERIODONTAL LIGAMENT CELLS IN VITRO
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Abstract
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-Abstract-
The responses of human periodontal ligament(PDL) fibroblastic cells to natural
PDGF-AB, and insulin-like growth factor-Ⅰ(IGF-Ⅰ) were examined in vitro using PDL
cells obtained from the periodontal ligament of human tooth. The mitogenic effect of
growth factors was assessed by measuring the MTT assay. The morphologic effect for
growth factors showed under the transmission electron microscopy.
The results as follows;
1. The mitogenic response of the PDGF-AB was not observed at concentrations lower
than 1 ng/㎖, while the growth factor at 10 ng/㎖ increased mitogenic effect by as
much as three fold compared to the control value.
2. IGF-Ⅰ also was strongly mitogenic but at much higher concentrations than observed
for the PDGF-AB. The mitogenic effect for IGF-Ⅰ was noviced at 10 ng/㎖ and
reached a maximum level above 100 ng/㎖.
3. The combination of IGF-Ⅰ and PDGF-AB at 10 ng/㎖ increased the mitogenic
response 3.5 fold, suggesting synergism. This was the highest mitogenic effect observed higher, than that of IGF-Ⅰalone at 100 ng/㎖.
4. Under the transmission electron microscope, The growth factor-treated cells
maintained their elongated shape and a high degree of cellular polarization. The large
cell body containing a well developed Golgi complex, a large number of profiles of
rough endoplasmic reticulum, and great numbers of mitochondria.
These findings suggest that the PDGF-AB and IGF-Ⅰ may have important roles in
promotion of PDL healing and consequently may be useful for clinical application in
periodontal regenerative procedures.
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KEYWORD
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